A spokesman for Canadian National Railway Co. says three locomotives and 10 railcars have derailed in the U.S.
Patrick Waldron says the derailment happened Sunday night in the town of Springer, Wisconsin.
He says two crew members were injured.
Local emergency officials and company officials are on the scene.
More than 18,000 litres of diesel fuel spilled from an engine, forcing more than 100 nearby homes to be evacuated.
A middle school is acting as a temporary shelter.
Preliminary reports indicate the southbound train struck another set of cars on another train.
There has been no official confirmation whether track conditions or speed played a factor in the derailment.