Martine Ouellet has lost a confidence vote on her leadership of the Bloc Quebecois.
The party says 32 per cent voted in favour of her leadership, whereas 67 per cent voted against.
The Bloc has been in disarray since late February when seven of its 10 members of Parliament quit over Ouellet's leadership style.
The Bloc's youth wing and one of the three remaining MPs also withdrew their support of Ouellet, who has been criticised for being uncompromising and for focusing too sharply on Quebec independence instead of defending the province's interests on the federal scene.