An accident on the 401 in the west end of the city closed the highway through the area for hours into early Saturday morning.
A transport trailer hauling live stock crashed in the express lanes near Hurontario at around 11:30 p.m.
The cattle on board managed to pry themselves loose and ended up running down the highway.
Video posted showed the cattle making their way down along the 401 before eventually crossing over to a grassy nole.
Both the express and collector lanes were fully reopened by 6 a.m. Saturday.
There was no word from the police on the cause of the crash or any injuries.
with files from CP24